Improved concordance of challenging human epidermal growth factor receptor 2 dual in-situ hybridisation cases with the use of a digital image analysis algorithm in breast cancer
Details
Publication Year 2023-10,Volume 83,Issue #4,Page 647-656
Journal Title
Histopathology
Publication Type
Research article
Abstract
AIMS: Accurate assessment of human epidermal growth factor receptor 2 (HER2) expression by HER2 immunohistochemistry and in-situ hybridisation (ISH) is critical for the management of patients with breast cancer. The revised 2018 ASCO/CAP guidelines define 5 groups based on HER2 expression and copy number. Manual pathologist quantification by light microscopy of equivocal and less common HER2 ISH groups (groups 2-4) can be challenging, and there are no data on interobserver variability in reporting of these cases. We sought to determine whether a digital algorithm could improve interobserver variability in the assessment of difficult HER2 ISH cases. METHODS AND RESULTS: HER2 ISH was evaluated in a cohort enriched for less common HER2 patterns using standard light microscopy versus analysis of whole slide images using the Roche uPath HER2 dual ISH image analysis algorithm. Standard microscopy demonstrated significant interobserver variability with a Fleiss's kappa value of 0.471 (fair-moderate agreement) improving to 0.666 (moderate-good) with the use of the algorithm. For HER2 group designation (groups 1-5), there was poor-moderate reliability between pathologists by microscopy [intraclass correlation coefficient (ICC) = 0.526], improving to moderate-good agreement (ICC = 0.763) with the use of the algorithm. In subgroup analysis, the algorithm improved concordance particularly in groups 2, 4 and 5. Time to enumerate cases was also significantly reduced. CONCLUSION: This work demonstrates the potential of a digital image analysis algorithm to improve the concordance of pathologist HER2 amplification status reporting in less common HER2 groups. This has the potential to improve therapy selection and outcomes for patients with HER2-low and borderline HER2-amplified breast cancers.
Publisher
Wiley
Keywords
Humans; Female; *Breast Neoplasms/genetics/metabolism; In Situ Hybridization, Fluorescence/methods; Reproducibility of Results; Receptor, ErbB-2/genetics/metabolism; Algorithms; Biomarkers, Tumor/metabolism; Erbb2; Her2; breast cancer; bright-field ISH; digital pathology; image analysis; in-situ hybridisation
Department(s)
Pathology
PubMed ID
37366040
Open Access at Publisher's Site
https://doi.org/10.1111/his.15000
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2023-11-30 06:20:59
Last Modified: 2023-11-30 06:21:26

© 2024 The Walter and Eliza Hall Institute of Medical Research. Access to this website is subject to our Privacy Policy and Terms of Use

An error has occurred. This application may no longer respond until reloaded. Reload 🗙