Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma
Journal Title
Scientific Reports
Publication Type
Research article
Abstract
The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was observed in 3D-ACC immune co-culture models. Gipie-silencing conferred a "lymphoblast-like" morphology to ACC cells, a six-fold increase in apoptotic cells (compared to unaltered ACC cells, P </= 0.0001), a two-fold decrease in T regulatory cells (FoxP3(+)/IL-2Ralpha(+)/CD25(+)) (P </= 0.0001), and a three-fold increase in activated NK cells (NKp30(+)/IFN-gamma(+)) (P </= 0.0001) with significantly higher release of granzyme (P </= 0.001) and perforin (P </= 0.0001).
Publisher
Springer Nature
Keywords
Humans; *Carcinoma, Adenoid Cystic/pathology; Killer Cells, Natural; T-Lymphocytes, Regulatory; Jurkat Cells; Perforin
Department(s)
Laboratory Research
PubMed ID
37813936
Open Access at Publisher's Site
https://doi.org/10.1038/s41598-023-44329-7
Terms of Use/Rights Notice
Refer to copyright notice on published article.


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Last Modified: 2023-11-30 03:03:40

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