Preclinical 3D-model supports an invisibility cloak for adenoid cystic carcinoma
- Author(s)
- Chakraborty, R; Darido, C; Chien, A; Tay, A; Vickery, K; Hu, H; Liu, F; Ranganathan, S;
- Journal Title
- Scientific Reports
- Publication Type
- Research article
- Abstract
- The tumour-cell based initiation of immune evasion project evaluated the role of Gipie in adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (A-253), from ninety-six 3D-ACC and A-253-immune co-culture models using natural killer cells (NK), and Jurkat cells (JK). Abnormal ACC morphology was observed in 3D-ACC immune co-culture models. Gipie-silencing conferred a "lymphoblast-like" morphology to ACC cells, a six-fold increase in apoptotic cells (compared to unaltered ACC cells, P </= 0.0001), a two-fold decrease in T regulatory cells (FoxP3(+)/IL-2Ralpha(+)/CD25(+)) (P </= 0.0001), and a three-fold increase in activated NK cells (NKp30(+)/IFN-gamma(+)) (P </= 0.0001) with significantly higher release of granzyme (P </= 0.001) and perforin (P </= 0.0001).
- Publisher
- Springer Nature
- Keywords
- Humans; *Carcinoma, Adenoid Cystic/pathology; Killer Cells, Natural; T-Lymphocytes, Regulatory; Jurkat Cells; Perforin
- Department(s)
- Laboratory Research
- PubMed ID
- 37813936
- Publisher's Version
- https://doi.org/10.1038/s41598-023-44329-7
- Open Access at Publisher's Site
https://doi.org/10.1038/s41598-023-44329-7- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-11-30 02:33:20
Last Modified: 2023-11-30 03:03:40