Circulating microbial cell-free DNA is increased during neutropenia after hematopoietic stem cell transplantation
- Author(s)
- Blair, LM; Akhund-Zade, J; Katsamakis, ZA; Smibert, OC; Wolfe, AE; Giardina, P; Slingerland, J; Bercovici, S; Perales, MA; Taur, Y; van den Brink, MRM; Peled, JU; Markey, KA;
- Details
- Publication Year 2023,Volume 7,Issue #21,Page 6744-6750
- Journal Title
- Blood Advances
- Publication Type
- Research article
- Abstract
- We used a next-generation sequencing platform to characterize microbial cell-free DNA (mcfDNA) in plasma samples from patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HCT). In this observational study, we sought to characterize plasma mcfDNA in order to explore its potential association with the immunologic complications of transplantation. We compared serially collected patient samples with plasma collected from healthy control subjects. We observed changes in total mcfDNA burden in the plasma after transplantation, which was most striking during the early posttransplant neutropenic phase. This elevation could be attributed to a number of specific bacterial taxa, including Veillonella, Bacteroides, and Prevotella (genus level). For an additional cohort of patients, we compared the data of mcfDNA from plasma with 16s-ribosomal RNA sequencing data from stool samples collected at matched time points. In a number of patients, we confirmed that mcfDNA derived from specific microbial taxa (eg, Enterococcus) could also be observed in the matched stool sample. Quantification of mcfDNA may generate novel insights into mechanisms by which the intestinal microbiome influences systemic cell populations and, thus, has been associated with outcomes for patients with cancer.
- Publisher
- American Society of Hematology
- Keywords
- Humans; *Cell-Free Nucleic Acids; *Graft vs Host Disease/etiology; *Hematopoietic Stem Cell Transplantation/adverse effects; *Neoplasms/complications; *Neutropenia
- Department(s)
- Infectious Diseases
- PubMed ID
- 37399491
- Publisher's Version
- https://doi.org/10.1182/bloodadvances.2023010208
- Open Access at Publisher's Site
- https://doi.org/10.1182/bloodadvances.2023010208
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-11-30 02:33:19
Last Modified: 2023-11-30 03:03:40