A spatially localised cryoinjury protocol to study liver regeneration in zebrafish
- Author(s)
- Sande-Melon, M; Logan, BK; Cox, AG; González-Rosa, JM;
- Details
- Publication Year 2026-03-20,Volume 7,Issue #1,Page 104420
- Journal Title
- STAR Protocols
- Publication Type
- Protocol
- Abstract
- Here, we present a protocol for studying liver regeneration in adult zebrafish using a hepatic cryoinjury technique. We detail the surgical procedure to induce localized necrosis using a cryoprobe. We then detail steps for liver harvesting and CUBIC-based tissue clearing for three-dimensional imaging. The protocol enables comparison of healthy and injured liver parenchyma within individual animals, recapitulating key features of human chronic liver disease, including fibrosis formation and resolution.
- Publisher
- Cell Press
- Keywords
- Animals; *Zebrafish/physiology; *Liver Regeneration/physiology; *Liver/injuries/pathology; Imaging, Three-Dimensional/methods; Developmental biology; Microscopy; Model organisms; Stem cells
- Department(s)
- Laboratory Research
- Publisher's Version
- https://doi.org/10.1016/j.xpro.2026.104420
- Open Access at Publisher's Site
https://doi.org/10.1016/j.xpro.2026.104420- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2026-04-07 03:20:36
Last Modified: 2026-04-07 03:20:52