T cell and monocyte activation in concert with hematopoietic stem cell interactions shapes the post-allogeneic transplant immune landscape in poor graft function
- Author(s)
- Prabahran, A; Wu, Z; Gao, S; Morgan, H; Holzwart, N; Ludford-Menting, M; Rawicki, M; Klass, J; Koo, RM; Wilson, C; Blombery, P; Tan, CW; Indukuri, S; Chee, L; Ritchie, D; Young, NS; Feng, X; Koldej, R;
- Journal Title
- Frontiers in Immunology
- Publication Type
- Research article
- Abstract
- INTRODUCTION: Post-allogeneic stem cell transplantation (alloSCT) can be complicated by poor graft function (PGF), a life-threatening condition characterized by complete donor chimerism alongside persistent multilineage cytopenias. PGF significantly increases the risk of bleeding, infection, and transfusion dependence. The cellular changes during hematopoiesis post-alloSCT, particularly in PGF, remain poorly defined. METHODS: To evaluate the immune and hematopoietic reconstitution and dysfunction post-alloSCT, with a focus on PGF, we applied a comprehensive suite of histological, immunological, and molecular biological techniques to bone marrow (BM) and peripheral blood samples from patients with PGF, good graft function (GGF), and healthy donors (HDs). RESULTS: By approximately 100 days post-alloSCT, patients demonstrated T cell oligoclonality, activation, and exhaustion compared to HDs. BM nucleated cells, particularly monocytes, exhibited increased activation and IFN-g response post-alloSCT compared to those of HDs. Moreover, cell-cell interactions between immune cells and hematopoietic stem and progenitor cells were notably enhanced post-alloSCT. While most inflammatory changes were present in both PGF and GGF, they were more pronounced in PGF. DISCUSSION: Our results demonstrate a hyper-inflamed post-alloSCT environment involving both innate (monocytes) and adaptive (T cells) immune responses and their active interactions, more in PGF, highlighting that immune modulation may serve as an alternative or adjunctive therapeutic approach for PGF.
- Publisher
- Frontiers
- Keywords
- Humans; *Hematopoietic Stem Cell Transplantation/adverse effects; *Monocytes/immunology/metabolism; *Hematopoietic Stem Cells/immunology/metabolism; Male; *T-Lymphocytes/immunology/metabolism; Female; Middle Aged; Adult; Transplantation, Homologous; *Cell Communication/immunology; *Lymphocyte Activation/immunology; Young Adult; Aged; T cell receptor; hematopoietic cell transplantation; immune cell interactions; immune reconstitution; single-cell RNA sequence
- Department(s)
- Haematology; Pathology
- Publisher's Version
- https://doi.org/10.3389/fimmu.2026.1750093
- Open Access at Publisher's Site
https://doi.org/10.3389/fimmu.2026.1750093- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2026-03-10 04:07:04
Last Modified: 2026-03-10 04:07:14