Glial Hedgehog signalling and lipid metabolism regulate neural stem cell proliferation in Drosophila
- Author(s)
- Dong, Q; Zavortink, M; Froldi, F; Golenkina, S; Lam, T; Cheng, LY;
- Details
- Publication Year 2021-05,Volume 22,Issue #5,Page e52130
- Journal Title
- EMBO Reports
- Publication Type
- Research article
- Abstract
- The final size and function of the adult central nervous system (CNS) are determined by neuronal lineages generated by neural stem cells (NSCs) in the developing brain. In Drosophila, NSCs called neuroblasts (NBs) reside within a specialised microenvironment called the glial niche. Here, we explore non-autonomous glial regulation of NB proliferation. We show that lipid droplets (LDs) which reside within the glial niche are closely associated with the signalling molecule Hedgehog (Hh). Under physiological conditions, cortex glial Hh is autonomously required to sustain niche chamber formation. Upon FGF-mediated cortex glial overgrowth, glial Hh non-autonomously activates Hh signalling in the NBs, which in turn disrupts NB cell cycle progression and its ability to produce neurons. Glial Hh's ability to signal to NB is further modulated by lipid storage regulator lipid storage droplet-2 (Lsd-2) and de novo lipogenesis gene fatty acid synthase 1 (Fasn1). Together, our data suggest that glial-derived Hh modified by lipid metabolism mechanisms can affect the neighbouring NB's ability to proliferate and produce neurons.
- Keywords
- Animals; Cell Proliferation; Drosophila/metabolism; *Drosophila Proteins/genetics/metabolism; Drosophila melanogaster/genetics/metabolism; Hedgehog Proteins/genetics; Lipid Metabolism; *Neural Stem Cells/metabolism
- Department(s)
- Laboratory Research
- PubMed ID
- 33751817
- Publisher's Version
- https://doi.org/10.15252/embr.202052130
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2026-01-30 05:59:57
Last Modified: 2026-01-30 06:01:39