Society for Immunotherapy of Cancer: Standards for Reporting of Multiplex Immunohistochemistry/Immunofluorescence Assays (STORMI)

Sater, S; Bifulco, CB; Rodriguez-Canales, J; Yeong, J; Akturk, G; Angelo, M; Ballesteros-Merino, C; Bankhead, P; Basu, S; Blando, JM; Brajkovic, S; Cassano, M; Chen, BJ; Coskun, AF; Cottrell, TR; de Andrea, CE; Edwards, RH; Egelston, C; Engle, LL; Ernstoff, MS; Fan, R; Feldman, M; Fox, BA; Galon, J; Gartrell, R; Gnjatic, S; Green, BF; Gulley, JL; Hellebust, A; Hewitt, S; Hollmann, TJ; Horn, LA; Howat, WJ; Hoyt, CC; Jensen, SM; Kulasinghe, A; Lassoued, W; Lott, S; Mansfield, J; Marwitz, S; Netto, G; Page, DB; Parra, E; Rimm, DL; Rodig, SJ; Salgado, R; Schapiro, D; Schalper, KA; Sunshine, JC; Surace, MJ; Szalay, AS; Thurin, M; Villasboas, JC; Wharton, K; Wistuba, II; Yearley, JH; Yuan, Y; Zaki, G; Ziai, J; Taube, JM

Author(s): Sater, S; Bifulco, CB; Rodriguez-Canales, J; Yeong, J; Akturk, G; Angelo, M; Ballesteros-Merino, C; Bankhead, P; Basu, S; Blando, JM; Brajkovic, S; Cassano, M; Chen, BJ; Coskun, AF; Cottrell, TR; de Andrea, CE; Edwards, RH; Egelston, C; Engle, LL; Ernstoff, MS; Fan, R; Feldman, M; Fox, BA; Galon, J; Gartrell, R; Gnjatic, S; Green, BF; Gulley, JL; Hellebust, A; Hewitt, S; Hollmann, TJ; Horn, LA; Howat, WJ; Hoyt, CC; Jensen, SM; Kulasinghe, A; Lassoued, W; Lott, S; Mansfield, J; Marwitz, S; Netto, G; Page, DB; Parra, E; Rimm, DL; Rodig, SJ; Salgado, R; Schapiro, D; Schalper, KA; Sunshine, JC; Surace, MJ; Szalay, AS; Thurin, M; Villasboas, JC; Wharton, K; Wistuba, II; Yearley, JH; Yuan, Y; Zaki, G; Ziai, J; Taube, JM;
Details: Publication Year 2025-12-21,Volume 13,Issue #12,Page e012280
Journal Title: Journal for ImmunoTherapy of Cancer
Publication Type: Guideline
Abstract: Multiplex immunofluorescence and immunohistochemistry (mIF/IHC) are increasingly employed antibody-based technologies that use tissue sparingly and facilitate the detection of co-localized or neighboring biomarkers. Specifically, these platforms enable spatial analyses of the tumor microenvironment as well as extended applications, for example, describing normal tissue anatomy, autoimmunity, infectious diseases, etc. mIF/IHC has greatly enhanced biomarker discovery efforts, and a growing number of studies suggest superiority to traditional IHC. Standardization of staining approaches, reporting of image analysis strategies and resultant data is critical for facilitating cross-study comparisons, validation, deployment, and generalization of findings. To address this challenge, The Society for Immunotherapy of Cancer (SITC) previously published two articles providing best practice guidelines for mIF/IHC staining, image analysis, and data sharing. Here, SITC convened stakeholders to develop the third article in the series, a consensus checklist for scientific reporting of mIF/IHC data to support and complement the best practice guidelines. The checklist includes critical components of mIF/IHC applications to be defined within publications such as detailed descriptions of analytical validation; image acquisition, selection, and registration methods; and cell clustering and spatial analysis strategies, amongst others. Such information will help with data reproducibility and comparison across studies towards future drug and assay development.
Keywords: Humans; *Immunohistochemistry/methods/standards; *Neoplasms/immunology/therapy/diagnosis; *Immunotherapy/methods/standards; *Fluorescent Antibody Technique/methods/standards; Tumor Microenvironment; *Biomarkers, Tumor; Biomarker; Immunotherapy; Pathology; Tumor microenvironment - TME
Department(s): Laboratory Research
Publisher's Version: https://doi.org/10.1136/jitc-2025-012280
Open Access at Publisher's Site: https://doi.org/10.1136/jitc-2025-012280
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2026-01-23 03:46:45

Last Modified: 2026-01-23 03:52:13