Single-cell protein expression profiling resolves circulating and resident memory T cell diversity across tissues and infection contexts
- Author(s)
- Evrard, M; Becht, E; Fonseca, R; Obers, A; Park, SL; Ghabdan-Zanluqui, N; Schroeder, J; Christo, SN; Schienstock, D; Lai, J; Burn, TN; Clatch, A; House, IG; Beavis, P; Kallies, A; Ginhoux, F; Mueller, SN; Gottardo, R; Newell, EW; Mackay, LK;
- Details
- Publication Year 2023-07-11,Volume 56,Issue #7,Page 1664-1680 e9
- Journal Title
- Immunity
- Publication Type
- Research article
- Abstract
- Memory CD8(+) T cells can be broadly divided into circulating (T(CIRCM)) and tissue-resident memory T (T(RM)) populations. Despite well-defined migratory and transcriptional differences, the phenotypic and functional delineation of T(CIRCM) and T(RM) cells, particularly across tissues, remains elusive. Here, we utilized an antibody screening platform and machine learning prediction pipeline (InfinityFlow) to profile >200 proteins in T(CIRCM) and T(RM) cells in solid organs and barrier locations. High-dimensional analyses revealed unappreciated heterogeneity within T(CIRCM) and T(RM) cell lineages across nine different organs after either local or systemic murine infection models. Additionally, we demonstrated the relative effectiveness of strategies allowing for the selective ablation of T(CIRCM) or T(RM) populations across organs and identified CD55, KLRG1, CXCR6, and CD38 as stable markers for characterizing memory T cell function during inflammation. Together, these data and analytical framework provide an in-depth resource for memory T cell classification in both steady-state and inflammatory conditions.
- Publisher
- Cell Press
- Keywords
- Mice; Animals; *CD8-Positive T-Lymphocytes; *Memory T Cells; Cell Lineage; Immunologic Memory; T cell memory; surface protein atlas; tissue immunity; tissue-resident memory T cells
- Department(s)
- Laboratory Research
- PubMed ID
- 37392736
- Publisher's Version
- https://doi.org/10.1016/j.immuni.2023.06.005
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-09-14 03:09:10
Last Modified: 2024-07-09 05:24:20