Duplex-Specific DNase Signal Amplification Allows Attomolar Electrochemical Detection of MicroRNAs
- Author(s)
- Pithaih, V; Bellette, HFF; Pimenta, TC; Pires Souto, DE; Tothill, RW; Punyadeera, C; Greene, GW; Hogan, CF; Abbey, B; Silva, SM;
- Journal Title
- Small
- Publication Type
- Research article
- Abstract
- Point-of-care biosensors for detecting blood-circulating nucleic acids hold significant potential for disease diagnosis. However, achieving both high sensitivity and accessibility remains a challenge. Electrochemical hybridization assays offer a promising approach for detecting specific nucleic acid sequences but are typically limited to picomolar concentrations. Here, a duplex-specific DNase-assisted electrochemical hybridization assay is presented, enabling highly sensitive nucleic acid detection. By incorporating duplex DNase, the method achieves detection limits as low as 1 attomolar, significantly enhancing sensitivity. This work paves the way for ultrasensitive nucleic acid detection in critical point-of-care applications.
- Publisher
- Wiley
- Keywords
- DNase‐assisted hybridization; electrochemistry; miRNA; point‐of‐care biosensor; signal amplification
- Department(s)
- Laboratory Research
- Publisher's Version
- https://doi.org/10.1002/smll.202507997
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2026-01-08 04:40:51
Last Modified: 2026-01-08 04:41:02