Analysis of the Radiosensitivity Index in Paired Preneoadjuvant and Postneoadjuvant Therapy Triple-Negative Breast Cancer

Stecklein, SR; Salgado, R; White, JR; Kimler, BF; Yoder, R; Staley, JM; O&#39;Dea, AP; Nye, LE; Satelli, D; Crane, GJ; Madan, R; O&#39;Neil, MF; Godwin, AK; Pathak, H; Khan, QJ; O&#39;Shaughnessy, J; Sharma, P

Author(s): Stecklein, SR; Salgado, R; White, JR; Kimler, BF; Yoder, R; Staley, JM; O'Dea, AP; Nye, LE; Satelli, D; Crane, GJ; Madan, R; O'Neil, MF; Godwin, AK; Pathak, H; Khan, QJ; O'Shaughnessy, J; Sharma, P;
Details: Publication Year 2026-03-01,Volume 124,Issue #3,Page 717-725
Journal Title: International Journal of Radiation Oncology, Biology, Physics
Publication Type: Research article
Abstract: PURPOSE: The radiosensitivity index (RSI) is a validated gene expression-based biomarker that can predict intrinsic radiosensitivity and has been shown to be associated with local control in patients with triple-negative breast cancer (TNBC) treated with upfront surgery. Currently, most patients with TNBC receive neoadjuvant systemic therapy (NAST). Whether the RSI predicts response to NAST and how the RSI and predicted radiosensitivity are altered by exposure to NAST is unknown. METHODS AND MATERIALS: Total RNA was extracted from pretreatment core needle biopsy specimens from 197 patients with TNBC treated on the NeoSTOP (NCT02413320) and NeoPACT (NCT03639948) trials. Total RNA was also extracted from paired post-NAST (residual disease) tumor tissue in 58 patients. A published algorithm using 10 genes was used to compute the RSI for each tumor. Stromal tumor-infiltrating lymphocytes (sTILs) were scored on pretreatment and post-NAST samples by an expert breast pathologist according to international consensus guidelines. CIBERSORTx was used to impute leukocyte fractions in samples using RNA-sequencing data. RESULTS: Cluster analysis of RSI genes in pretreatment samples revealed immune-depleted and immune-enriched groups, and this classification was strongly associated with sTIL infiltration (P < .0001) and likelihood of achieving pathologic complete response (pCR) (P = .001). RSI showed associations (false discovery rate q < 0.01) with M0 and M1 macrophages, CD4+ memory resting, CD4+ memory activated, CD8+, and follicular helper T-cells, activated natural killer cells, naïve and memory B cells, and resting dendritic cells on CIBERSORTx leukocyte deconvolution. In the entire cohort, NAST-induced change in RSI was variable, but among initially RSI-immune-enriched tumors that did not achieve pCR, there was a significant decrease in predicted radiosensitivity between paired pretreatment and post-NAST samples. NAST-induced reduction in sTILs and naïve B cells may be associated with this decrease in radiosensitivity. CONCLUSIONS: Pretreatment RSI cluster identity is associated with the degree of immune enrichment and response to NAST in TNBC. Initially immune-enriched TNBCs that do not achieve a pCR to NAST exhibit a decrease in predicted radiosensitivity compared with paired pretreatment tumors.
Publisher: Elsevier
Keywords: Humans; *Triple Negative Breast; Neoplasms/radiotherapy/genetics/pathology/immunology/therapy; *Neoadjuvant Therapy; Female; *Radiation Tolerance/genetics; Middle Aged; Lymphocytes, Tumor-Infiltrating; Aged; Adult; Algorithms
Department(s): Laboratory Research
Publisher's Version: https://doi.org/10.1016/j.ijrobp.2025.09.038
Open Access at Publisher's Site: https://doi.org/10.1016/j.ijrobp.2025.09.038
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2025-10-23 05:42:31

Last Modified: 2026-03-02 05:02:25