Genome-wide analysis of host-encoded microRNAs modulating SARS-CoV-2 infection

Rootes, CL; Cowley, KJ; Brice, AM; Beetham, HG; Sathiqu, RM; Simpson, KJ; Stewart, CR

Author(s): Rootes, CL; Cowley, KJ; Brice, AM; Beetham, HG; Sathiqu, RM; Simpson, KJ; Stewart, CR;
Details: Publication Year 2025-07-31,Volume 12,Issue #1,Page 1330
Journal Title: Scientific Data
Publication Type: Research article
Abstract: Viruses exploit cellular machinery to complete their replication cycle. Furthering our understanding of this process provides insight into the mechanism of virus replication and potential targets for antiviral therapeutics. Genome-wide CRISPR screens have identified cellular pathways important in the SARS-COV-2 infection process, including vesicular traffic, lipid homeostasis and PI3K signalling. Functional genomics-driven analysis of host-encoded microRNAs (miRNAs) impacting SARS-CoV-2 infection would provide further unbiased and discovery-driven insight into the host-pathogen interface. Here we present findings from genome-wide complementary miRNA mimic and inhibitor screens performed in a bio-safety level (BSL)-4 laboratory using a combination of high-throughput robotics, high-content imaging and novel data analysis pipelines. This dataset has identified both miRNA promoters and inhibitors of SARS-CoV-2 replication which may be used by researchers to further explore therapeutic targets against SARS-CoV-2 and the host factors influencing COVID pathogenesis.
Publisher: Springer Nature
Keywords: *MicroRNAs/genetics; *COVID-19/genetics/virology; Humans; *SARS-CoV-2/physiology; Virus Replication/genetics; *Host-Pathogen Interactions/genetics; Genome-Wide Association Study
Department(s): Laboratory Research
Publisher's Version: https://doi.org/10.1038/s41597-025-05669-3
Open Access at Publisher's Site: https://doi.org/10.1038/s41597-025-05669-3
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2025-08-26 05:34:24

Last Modified: 2025-08-26 05:34:30