Relative importance of the anti-apoptotic versus apoptosis-unrelated functions of MCL-1 in vivo
Journal Title
Science
Publication Type
Online publication before print
Abstract
The anti-apoptotic protein MCL-1 (myeloid cell leukemia-1) is essential for embryogenesis and the survival of many cell types that tolerate loss of its relatives, BCL-XL and BCL-2. Apoptosis-unrelated roles of MCL-1 in metabolism may contribute to this requirement, though their relevance for embryogenesis and postnatal life remains unclear. We hypothesized that BCL-XL and BCL-2 may substitute MCL-1's anti-apoptotic but not its apoptosis-unrelated functions. Replacing MCL-1 with BCL-XL or BCL-2 supported embryo development by rescuing the Mcl-1(-/-) preimplantation lethality. Mcl-1(Bcl-xL/Bcl-xL) but not Mcl-1(Bcl-2/Bcl-2) mice were born on a mixed background, though they showed metabolic defects. Thus MCL-1's apoptosis-unrelated functions appear critical in later development, with BCL-XL, but not BCL-2, partially compensating. These findings clarify MCL-1's distinct physiological roles, critically informing MCL-1 inhibitor development as cancer therapeutics.
Department(s)
Laboratory Research
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Creation Date: 2025-07-22 03:31:00
Last Modified: 2025-07-22 03:31:16
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