Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation

Chakraborty, R; Vickery, K; Darido, C; Ranganathan, S; Hu, H

Author(s): Chakraborty, R; Vickery, K; Darido, C; Ranganathan, S; Hu, H;
Details: Publication Year 2021-08-12,Volume 22,Issue #16,Page 8686
Journal Title: International Journal of Molecular Sciences
Publication Type: Research article
Abstract: Oral cancer is a major global health problem with high incidence and low survival rates. The oral cavity contains biofilms as dental plaques that harbour both Gram-negative and Gram-positive bacterial antigens, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), respectively. LPS and LTA are known to stimulate cancer cell growth, and the bioactive phytochemical capsaicin has been reported to reverse this effect. Here, we tested the efficacy of oral cancer chemotherapy treatment with capsaicin in the presence of LPS, LTA or the combination of both antigens. LPS and LTA were administered to Cal 27 oral cancer cells prior to and/or concurrently with capsaicin, and the treatment efficacy was evaluated by measuring cell proliferation and apoptotic cell death. We found that while capsaicin inhibits oral cancer cell proliferation and metabolism (MT Glo assay) and increases cell death (Trypan blue exclusion assay and Caspase 3/7 expression), its anti-cancer effect was significantly reduced on cells that are either primed or exposed to the bacterial antigens. Capsaicin treatment significantly increased oral cancer cells' suppressor of cytokine signalling 3 gene expression. This increase was reversed in the presence of bacterial antigens during treatment. Our data establish a rationale for clinical consideration of bacterial antigens that may interfere with the treatment efficacy of oral cancer.
Keywords: Antigens, Bacterial/*adverse effects; Capsaicin/*pharmacology; Caspase 3/metabolism; Caspase 7/metabolism; Cell Line, Tumor; Cell Proliferation/drug effects; Cell Survival/drug effects; Gene Expression Regulation, Neoplastic/drug effects; Humans; Lipopolysaccharides/adverse effects; Mouth Neoplasms/drug therapy/*metabolism/microbiology; Signal Transduction/*drug effects; Teichoic Acids/adverse effects
Department(s): Laboratory Research
PubMed ID: 34445392
Publisher's Version: https://doi.org/10.3390/ijms22168686
Open Access at Publisher's Site: https://doi.org/10.3390/ijms22168686
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2025-06-20 08:03:12

Last Modified: 2025-06-20 08:04:25