Fully Automated 68Ga-Labeling and Purification of Macroaggregated Albumin Particles for Lung Perfusion PET Imaging
- Author(s)
- Blanc-Beguin, F; Masset, J; Robin, P; Tripier, R; Hennebicq, S; Guilloux, V; Vriamont, C; Warnier, C; Cogulet, V; Eu, P; Salaun, PY; Le Roux, PY;
- Journal Title
- Frontiers in Nuclear Medicine
- Publication Type
- Research article
- Abstract
- Lung PET/CT is a promising imaging modality for regional lung function assessment. Our aim was to develop and validate a fast, simple, and fully automated GMP compliant [(68)Ga]Ga-MAA labeling procedure, using a commercially available [(99m)Tc]Tc-MAA kit, a direct gallium-68 eluate and including a purification of the [(68)Ga]Ga-MAA. Method: The synthesis parameters (pH, heating temperature) were manually determined. Automated (68)Ga-labeling of MAA was then developed on a miniAIO (Trasis(R), Ans, Belgium) module. An innovative automated process was developed for the purification. The process was then optimized and adapted to automate both the [(68)Ga]Ga-MAA synthesis and the isolation of gallium-68 eluate required for the pulmonary ventilation PET/CT. Results: The 15-min process demonstrated high reliability and reproducibility, with high synthesis yield (>95 %). Mean [(68)Ga]Ga-MAA radiochemical purity was 99 % +/- 0.6 %. The (68)Ga-labeled MAA particles size and morphology remained unchanged. Conclusion: A fast, user friendly, and fully automated process to produce GMP [(68)Ga]Ga-MAA for clinical use was developed. This automated process combining the advantages of using a non-modified MAA commercial kit, a gallium-68 eluate without pre-purification and an efficient final purification of the [(68)Ga]Ga-MAA may facilitate the implementation of lung PET/CT imaging in nuclear medicine departments.
- Keywords
- [68Ga]Ga-MAA; automated purification; automated synthesis; gallium-68; perfusion PET/CT
- Department(s)
- Cancer Imaging
- PubMed ID
- 39355632
- Publisher's Version
- https://doi.org/10.3389/fnume.2021.778191
- Open Access at Publisher's Site
https://doi.org/10.3389/fnume.2021.778191- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2025-06-13 06:47:57
Last Modified: 2025-06-13 06:48:45