The site of breast cancer metastases dictates their clonal composition and reversible transcriptomic profile
Details
Publication Year 2021-07,Volume 7,Issue #28,Page eabf4408
Journal Title
Science Advances
Publication Type
Research article
Abstract
Intratumoral heterogeneity is a driver of breast cancer progression, but the nature of the clonal interactive network involved in this process remains unclear. Here, we optimized the use of optical barcoding to visualize and characterize 31 cancer subclones in vivo. By mapping the clonal composition of thousands of metastases in two clinically relevant sites, the lungs and liver, we found that metastases were highly polyclonal in lungs but not in the liver. Furthermore, the transcriptome of the subclones varied according to their metastatic niche. We also identified a reversible niche-driven signature that was conserved in lung and liver metastases collected during patient autopsies. Among this signature, we found that the tumor necrosis factor-alpha pathway was up-regulated in lung compared to liver metastases, and inhibition of this pathway affected metastasis diversity. These results highlight that the cellular and molecular heterogeneity observed in metastases is largely dictated by the tumor microenvironment.
Keywords
*Breast Neoplasms/genetics/pathology; Female; Humans; *Liver Neoplasms/genetics/pathology; *Lung Neoplasms/pathology; Neoplasm Metastasis; Transcriptome; Tumor Microenvironment/genetics
Department(s)
Laboratory Research
PubMed ID
34233875
Open Access at Publisher's Site
https://doi.org/10.1126/sciadv.abf4408
Terms of Use/Rights Notice
Refer to copyright notice on published article.


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