Genomic and Transcriptomic Analyses of Breast Cancer Primaries and Matched Metastases in AURORA, the Breast International Group (BIG) Molecular Screening Initiative

Aftimos, P; Oliveira, M; Irrthum, A; Fumagalli, D; Sotiriou, C; Gal-Yam, EN; Robson, ME; Ndozeng, J; Di Leo, A; Ciruelos, EM; de Azambuja, E; Viale, G; Scheepers, ED; Curigliano, G; Bliss, JM; Reis-Filho, JS; Colleoni, M; Balic, M; Cardoso, F; Albanell, J; Duhem, C; Marreaud, S; Romagnoli, D; Rojas, B; Gombos, A; Wildiers, H; Guerrero-Zotano, A; Hall, P; Bonetti, A; Larsson, KF; Degiorgis, M; Khodaverdi, S; Greil, R; Sverrisdottir, A; Paoli, M; Seyll, E; Loibl, S; Linderholm, B; Zoppoli, G; Davidson, NE; Johannsson, OT; Bedard, PL; Loi, S; Knox, S; Cameron, DA; Harbeck, N; Montoya, ML; Brandao, M; Vingiani, A; Caballero, C; Hilbers, FS; Yates, LR; Benelli, M; Venet, D; Piccart, MJ

Author(s): Aftimos, P; Oliveira, M; Irrthum, A; Fumagalli, D; Sotiriou, C; Gal-Yam, EN; Robson, ME; Ndozeng, J; Di Leo, A; Ciruelos, EM; de Azambuja, E; Viale, G; Scheepers, ED; Curigliano, G; Bliss, JM; Reis-Filho, JS; Colleoni, M; Balic, M; Cardoso, F; Albanell, J; Duhem, C; Marreaud, S; Romagnoli, D; Rojas, B; Gombos, A; Wildiers, H; Guerrero-Zotano, A; Hall, P; Bonetti, A; Larsson, KF; Degiorgis, M; Khodaverdi, S; Greil, R; Sverrisdottir, A; Paoli, M; Seyll, E; Loibl, S; Linderholm, B; Zoppoli, G; Davidson, NE; Johannsson, OT; Bedard, PL; Loi, S; Knox, S; Cameron, DA; Harbeck, N; Montoya, ML; Brandao, M; Vingiani, A; Caballero, C; Hilbers, FS; Yates, LR; Benelli, M; Venet, D; Piccart, MJ;
Details: Publication Year 2021-11,Volume 11,Issue #11,Page 2796-2811
Journal Title: Cancer Discovery
Publication Type: Research article
Abstract: AURORA aims to study the processes of relapse in metastatic breast cancer (MBC) by performing multi-omics profiling on paired primary tumors and early-course metastases. Among 381 patients (primary tumor and metastasis pairs: 252 targeted gene sequencing, 152 RNA sequencing, 67 single nucleotide polymorphism arrays), we found a driver role for GATA1 and MEN1 somatic mutations. Metastases were enriched in ESR1, PTEN, CDH1, PIK3CA, and RB1 mutations; MDM4 and MYC amplifications; and ARID1A deletions. An increase in clonality was observed in driver genes such as ERBB2 and RB1. Intrinsic subtype switching occurred in 36% of cases. Luminal A/B to HER2-enriched switching was associated with TP53 and/or PIK3CA mutations. Metastases had lower immune score and increased immune-permissive cells. High tumor mutational burden correlated to shorter time to relapse in HR(+)/HER2(-) cancers. ESCAT tier I/II alterations were detected in 51% of patients and matched therapy was used in 7%. Integration of multi-omics analyses in clinical practice could affect treatment strategies in MBC. SIGNIFICANCE: The AURORA program, through the genomic and transcriptomic analyses of matched primary and metastatic samples from 381 patients with breast cancer, coupled with prospectively collected clinical data, identified genomic alterations enriched in metastases and prognostic biomarkers. ESCAT tier I/II alterations were detected in more than half of the patients.This article is highlighted in the In This Issue feature, p. 2659.
Keywords: Biomarkers, Tumor/genetics; *Breast Neoplasms/diagnosis/drug therapy/genetics; Cell Cycle Proteins/genetics; Early Detection of Cancer; Female; Genomics; High-Throughput Nucleotide Sequencing; Humans; Mutation; Neoplasm Recurrence, Local/pathology; Proto-Oncogene Proteins/genetics; Transcriptome
Department(s): Medical Oncology; Laboratory Research
PubMed ID: 34183353
Publisher's Version: https://doi.org/10.1158/2159-8290.CD-20-1647
Open Access at Publisher's Site: https://doi.org/10.1158/2159-8290.CD-20-1647
Terms of Use/Rights Notice: Refer to copyright notice on published article.

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Last Modified: 2025-05-23 04:04:10