Assessing cancer therapeutic efficacy in vivo using [2H7]glucose deuterium metabolic imaging

Chang, MC; Malut, VR; Mahar, R; Rushin, A; McLeod, MA; Pierre, GL; Malut, IR; Staklinski, SJ; Glanz, ME; Ragavan, M; Sharma, G; Madheswaran, M; Badar, A; Rao, AD; Law, BK; Kilberg, MS; Collins, JHP; Kodibagkar, VD; Bankson, JA; Deberardinis, RJ; Merritt, ME

Author(s): Chang, MC; Malut, VR; Mahar, R; Rushin, A; McLeod, MA; Pierre, GL; Malut, IR; Staklinski, SJ; Glanz, ME; Ragavan, M; Sharma, G; Madheswaran, M; Badar, A; Rao, AD; Law, BK; Kilberg, MS; Collins, JHP; Kodibagkar, VD; Bankson, JA; Deberardinis, RJ; Merritt, ME;
Details: Publication Year 2025-03-28,Volume 11,Issue #13,Page eadr0568
Journal Title: Science Advances
Publication Type: Research article
Abstract: Metabolic imaging produces powerful visual assessments of organ function in vivo. Current techniques can be improved by safely increasing metabolic contrast. The gold standard, 2-[(18)F]fluorodeoxyglucose-positron emission tomography (FDG-PET) imaging, is limited by radioactive exposure and sparse assessment of metabolism beyond glucose uptake and retention. Deuterium magnetic resonance imaging (DMRI) with [6,6-(2)H(2)]glucose is nonradioactive, achieves tumor metabolic contrast, but can be improved by enriched contrast from deuterated water (HDO) based imaging. Here, we developed a DMRI protocol employing [(2)H(7)]glucose. Imaging (2)H-signal and measuring HDO production in tumor-bearing mice detected differential glucose utilization across baseline tumors, tumors treated with vehicle control or anti-glycolytic BRAFi and MEKi therapy, and contralateral healthy tissue. Control tumors generated the most (2)H-signal and HDO. To our knowledge this is the first application of DMRI with [(2)H(7)]glucose for tumoral treatment monitoring. This approach demonstrates HDO as a marker of tumor glucose utilization and suggests translational capability in humans due to its safety, noninvasiveness, and suitability for serial monitoring.
Publisher: American Association for the Advancement of Science
Keywords: Animals; *Glucose/metabolism; *Deuterium/chemistry; Mice; Humans; *Magnetic Resonance Imaging/methods; *Neoplasms/drug therapy/diagnostic imaging/metabolism; Cell Line, Tumor; Positron-Emission Tomography/methods; Fluorodeoxyglucose F18/chemistry; Treatment Outcome
Department(s): Medical Oncology
Publisher's Version: https://doi.org/10.1126/sciadv.adr0568
Open Access at Publisher's Site: https://doi.org/10.1126/sciadv.adr0568
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2025-04-10 03:35:22

Last Modified: 2025-04-10 03:35:32