Rapid Point-of-Care Electrochemical Sensor for the Detection of Cancer Tn Antigen Carbohydrate in Whole Unprocessed Blood
- Author(s)
- Silva, SM; Langley, DP; Cossins, LR; Samudra, AN; Quigley, AF; Kapsa, RMI; Tothill, RW; Greene, GW; Moulton, SE;
- Details
- Publication Year 2022-11-25,Volume 7,Issue #11,Page 3379-3388
- Journal Title
- ACS Sensors
- Publication Type
- Research article
- Abstract
- Improving outcomes for cancer patients during treatment and monitoring for cancer recurrence requires personalized care which can only be achieved through regular surveillance for biomarkers. Unfortunately, routine detection for blood-based biomarkers is cost-prohibitive using currently specialized laboratories. Using a rapid self-assembly sensing interface amenable to methods of mass production, we demonstrate the ability to detect and quantify a small carbohydrate-based cancer biomarker, Tn antigen (alphaGalNAc-Ser/Thr) in a small volume of blood, using a test format strip reminiscent of a blood glucose test. The detection of Tn antigen at picomolar levels is achieved through a new transduction mechanism based on the impact of Tn antigen interactions on the molecular dynamic motion of a lectin cross-linked lubricin antifouling brush. In tests performed on retrospective blood plasma samples from patients presenting three different tumor types, differentiation between healthy and diseased patients was achieved, highlighting the clinical potential for cancer monitoring.
- Keywords
- Humans; *Point-of-Care Systems; Retrospective Studies; *Neoplasms/diagnosis; Carbohydrates; Tn antigen; abnormal O-glycans; antifouling coatings; cancer biomarkers; lectin-based diagnostics; liquid biopsies; lubricin; point-of-care electrochemical sensor
- Department(s)
- Laboratory Research
- PubMed ID
- 36374944
- Publisher's Version
- https://doi.org/10.1021/acssensors.2c01460
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2025-04-04 05:25:01
Last Modified: 2025-04-04 05:39:07