Receptor CDCP1 is a potential target for personalized imaging and treatment of poor outcome HER2+, triple negative and metastatic ER+/HER2- breast cancers
- Author(s)
- Gough, M; Kwah, KK; Khan, T; Ghosh, S; Sun, B; Lee, CY; Sokolowski, KA; Tse, BW; Wickramasuriya, L; Ferguson, K; Rogers, R; Goh, JB; Fletcher, NL; Houston, ZH; Thurecht, KJ; Bray, LJ; Liu, C; Pyke, C; Lim, E; Snell, CE; He, Y; Hooper, JD; Kryza, T;
- Journal Title
- Clinical Cancer Research
- Publication Type
- Online publication before print
- Abstract
- PURPOSE: Receptor CUB-domain containing- protein 1 (CDCP1) was evaluated as a target for detection and treatment of breast cancer. EXPERIMENTAL DESIGN: CDCP1 expression was assessed immunohistochemically in tumors from 423 patients (119 triple-negative breast cancer (TNBC); 75 HER2+; 229 ER+/HER2- including 228 primary tumors, 229 lymph node and 47 distant metastases). Cell cytotoxicity induced in vitro by a CDCP1-targeting antibody-drug conjugate (ADC), consisting of the human/mouse chimeric antibody ch10D7 and the microtubule disruptor monomethyl auristatin E (MMAE), was quantified, including in combination with HER2-targeting ADC T-DM1. Detection of CDCP1-expressing primary and metastatic xenografts in mice was examined by PET-CT imaging using 89zirconium-labelled ch10D7 (89Zr-ch10D7). The impact of ch10D7-MMAE on tumor burden and survival in vivo, including in combination with T-DM1, was quantified in cell line and patient-derived xenograft mouse models. RESULTS: CDCP1 is expressed predominantly on the surface of malignant cells of 70% of TNBCs, 80% of HER2+ tumors, and increases in ER+/HER2- tumors from 44.9% in primary tumors to 56.4% in lymph node metastases and 74.3% in distant metastases. PET-CT imaging with 89Zr-ch10D7 is effective for the detection of primary and metastatic CDCP1-expressing TNBCs in mice. ADC ch10D7-MMAE kills CDCP1-expressing cells in vitro and controls primary and metastatic TNBC xenografts in mice, conferring significant survival advantages over chemotherapy. It compares favorably to T-DM1 in vivo, and ch10D7-MMAE combined with T-DM1 showed the most potent efficacy, markedly reducing tumor burden of CDCP1+/HER2+ xenografts and prolonging mouse survival, compared with T-DM1 or ch10D7. CONCLUSIONS: CDCP1-directed molecular imaging has potential to identify aggressive breast cancers for CDCP1-targeted treatment.
- Department(s)
- Pathology
- Publisher's Version
- https://doi.org/10.1158/1078-0432.Ccr-24-2865
- Open Access at Publisher's Site
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Creation Date: 2025-01-30 02:31:24
Last Modified: 2025-01-30 02:34:12