Discovery of a novel dual functional phenylpyrazole-styryl hybrid that induces apoptotic and autophagic cell death in bladder cancer cells
Journal Title
European Journal of Medicinal Chemistry
Publication Type
Research article
Abstract
Unpleasant side effects and resistance development remained the Achilles heel of chemotherapy. Since low tumor-selectivity and monotonous effect of chemotherapy are closely related to such bottleneck, targeting tumor-selective multi-functional anticancer agents may be an ideal strategy in the search of new safer drugs. Herein, we report the discovery of compound 21, a nitro-substituted 1,5-diphenyl-3-styryl-1H-pyrazole that possesses dual functional characteristics. The 2D- and 3D-culture-based studies revealed that 21 not only could induce ROS-independent apoptotic and EGFR/AKT/mTOR-mediated autophagic cell deaths in EJ28 cells simultaneously but also has the ability in inducing cell death at both proliferating and quiescent zones of EJ28 spheroids. The molecular modelling analysis showed that 21 possesses EGFR targeting capability as it forms stable interactions in the EGFR active site. Together with its good safety profile in the zebrafish-based model, the present study showed that 21 is promising and may lead to the discovery of tumor-selective multi-functional anti-cancer agents.
Publisher
Elsevier
Keywords
Animals; *Autophagic Cell Death; Zebrafish; Cell Line, Tumor; *Antineoplastic Agents/pharmacology; *Urinary Bladder Neoplasms; ErbB Receptors; Apoptosis; Autophagy
Department(s)
Laboratory Research
PubMed ID
37098306
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2023-07-27 08:03:04
Last Modified: 2023-07-27 08:03:33

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