Cytokines enhance human Vgamma9Vdelta2 T-cell TCR-dependent and TCR-independent effector functions
- Details
- Publication Year 2023-06,Volume 53,Issue #6,Page e2250220
- Journal Title
- European Journal of Immunology
- Publication Type
- Research article
- Abstract
- Vgamma9Vdelta2 T cells can recognize various molecules associated with cellular stress or transformation, providing a unique avenue for the treatment of cancers or infectious diseases. Nonetheless, Vgamma9Vdelta2 T-cell-based immunotherapies frequently achieve suboptimal efficacies in vivo. Enhancing the cytotoxic effector function of Vgamma9Vdelta2 T cells is one potential avenue through which the immunotherapeutic potential of this subset may be improved. We compared the use of four pro-inflammatory cytokines on the effector phenotype and functions of in vitro expanded Vgamma9Vdelta2 T cells, and demonstrated TCR-independent cytotoxicity mediated through CD26, CD16, and NKG2D, which could be further enhanced by IL-23, IL-18, and IL-15 stimulation throughout expansion. This work defines promising culture conditions that could improve Vgamma9Vdelta2 T-cell-based immunotherapies and furthers our understanding of how this subset might recognize and target transformed or infected cells.
- Publisher
- Wiley
- Keywords
- Humans; *Cytokines; T-Lymphocyte Subsets; Receptors, Antigen, T-Cell, gamma-delta; Lymphocyte Activation; *Neoplasms; TCR-independent cytotoxicity; Vgamma9Vdelta2 T cells; cellular activation; human gammadelta T cells; pro-inflammatory cytokines
- Department(s)
- Laboratory Research
- PubMed ID
- 36946072
- Publisher's Version
- https://doi.org/10.1002/eji.202250220
- Open Access at Publisher's Site
10.1002/eji.202250220- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-07-19 07:47:32
Last Modified: 2024-07-10 06:33:23