Cytokines enhance human Vgamma9Vdelta2 T-cell TCR-dependent and TCR-independent effector functions
Details
Publication Year 2023-06,Volume 53,Issue #6,Page e2250220
Journal Title
European Journal of Immunology
Publication Type
Research article
Abstract
Vgamma9Vdelta2 T cells can recognize various molecules associated with cellular stress or transformation, providing a unique avenue for the treatment of cancers or infectious diseases. Nonetheless, Vgamma9Vdelta2 T-cell-based immunotherapies frequently achieve suboptimal efficacies in vivo. Enhancing the cytotoxic effector function of Vgamma9Vdelta2 T cells is one potential avenue through which the immunotherapeutic potential of this subset may be improved. We compared the use of four pro-inflammatory cytokines on the effector phenotype and functions of in vitro expanded Vgamma9Vdelta2 T cells, and demonstrated TCR-independent cytotoxicity mediated through CD26, CD16, and NKG2D, which could be further enhanced by IL-23, IL-18, and IL-15 stimulation throughout expansion. This work defines promising culture conditions that could improve Vgamma9Vdelta2 T-cell-based immunotherapies and furthers our understanding of how this subset might recognize and target transformed or infected cells.
Publisher
Wiley
Keywords
Humans; *Cytokines; T-Lymphocyte Subsets; Receptors, Antigen, T-Cell, gamma-delta; Lymphocyte Activation; *Neoplasms; TCR-independent cytotoxicity; Vgamma9Vdelta2 T cells; cellular activation; human gammadelta T cells; pro-inflammatory cytokines
Department(s)
Laboratory Research
PubMed ID
36946072
Open Access at Publisher's Site
10.1002/eji.202250220
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2023-07-19 07:47:32
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