Differential location of NKT and MAIT cells within lymphoid tissue
- Author(s)
- Johnson, DN; Ruan, Z; Petley, EV; Devi, S; Holz, LE; Uldrich, AP; Mak, JYW; Hor, JL; Mueller, SN; McCluskey, J; Fairlie, DP; Darcy, PK; Beavis, PA; Heath, WR; Godfrey, DI;
- Journal Title
- Scientific Reports
- Publication Type
- Research article
- Abstract
- Natural Killer T (NKT) cells and Mucosal-Associated Invariant T (MAIT) cells are innate-like T cells that express semi-invariant alphabeta T cell receptors (TCRs) through which they recognise CD1d and MR1 molecules, respectively, in complex with specific ligands. These cells play important roles in health and disease in many organs, but their precise intra-organ location is not well established. Here, using CD1d and MR1 tetramer staining techniques, we describe the precise location of NKT and MAIT cells in lymphoid and peripheral organs. Within the thymus, NKT cells were concentrated in the medullary side of the corticomedullary junction. In spleen and lymph nodes, NKT cells were mainly localised within T cell zones, although following in vivo activation with the potent NKT-cell ligand alpha-GalCer, they expanded throughout the spleen. MAIT cells were clearly detectable in Valpha19 TCR transgenic mice and were rare but detectable in lymphoid tissue of non-transgenic mice. In contrast to NKT cells, MAIT cells were more closely associated with the B cell zone and red pulp of the spleen. Accordingly, we have provided an extensive analysis of the in situ localisation of NKT and MAIT cells and suggest differences between the intra-organ location of these two cell types.
- Keywords
- Animals; *Lymphoid Tissue/metabolism; Mice; Mice, Transgenic; *Mucosal-Associated Invariant T Cells/metabolism; *Natural Killer T-Cells/metabolism; Receptors, Antigen, T-Cell/metabolism; Receptors, Antigen, T-Cell, alpha-beta/genetics/metabolism
- Department(s)
- Laboratory Research
- PubMed ID
- 35260653
- Publisher's Version
- https://doi.org/10.1038/s41598-022-07704-4
- Open Access at Publisher's Site
https://doi.org/10.1038/s41598-022-07704-4- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-12-19 03:18:23
Last Modified: 2024-12-19 03:23:08