Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae

Author(s): Dark, C; Cheung, S; Cheng, LY;
Details: Publication Year 2022-03-18,Volume 3,Issue #1,Page 101230
Journal Title: STAR Protocols
Publication Type: Protocol
Abstract: Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications in Drosophila larvae. We detail the procedures for dissecting, staining, and imaging third instar Drosophila larval muscle fillets and fat body, and how to utilize FIJI macros for robust quantification of cachectic phenotypes in these dissected tissues. For complete details on the use and execution of this protocol, please refer to Lodge et al. (2021).
Keywords: Animals; *Cachexia/metabolism; *Drosophila; Fat Body/metabolism; Larva/metabolism; Muscles/metabolism; Phenotype; Developmental biology; Metabolism; Model Organisms
Department(s): Laboratory Research
PubMed ID: 35284841
Publisher's Version: https://doi.org/10.1016/j.xpro.2022.101230
Open Access at Publisher's Site: https://doi.org/10.1016/j.xpro.2022.101230
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2024-10-18 06:46:44

Last Modified: 2024-10-18 06:47:15