Pkd1 and Wnt5a genetically interact to control lymphatic vascular morphogenesis in mice

Chau, TCY; Baek, S; Coxam, B; Skoczylas, R; Rondon-Galeano, M; Bower, NI; Wainwright, EN; Stacker, SA; Cooper, HM; Koopman, PA; Lagendijk, AK; Harvey, NL; Francois, M; Hogan, BM

Author(s): Chau, TCY; Baek, S; Coxam, B; Skoczylas, R; Rondon-Galeano, M; Bower, NI; Wainwright, EN; Stacker, SA; Cooper, HM; Koopman, PA; Lagendijk, AK; Harvey, NL; Francois, M; Hogan, BM;
Details: Publication Year 2022-02,Volume 251,Issue #2,Page 336-349
Journal Title: Developmental Dynamics
Publication Type: Research article
Abstract: BACKGROUND: Lymphatic vascular development is regulated by well-characterized signaling and transcriptional pathways. These pathways regulate lymphatic endothelial cell (LEC) migration, motility, polarity, and morphogenesis. Canonical and non-canonical WNT signaling pathways are known to control LEC polarity and development of lymphatic vessels and valves. PKD1, encoding Polycystin-1, is the most commonly mutated gene in polycystic kidney disease but has also been shown to be essential in lymphatic vascular morphogenesis. The mechanism by which Pkd1 acts during lymphangiogenesis remains unclear. RESULTS: Here we find that loss of non-canonical WNT signaling components Wnt5a and Ryk phenocopy lymphatic defects seen in Pkd1 knockout mice. To investigate genetic interaction, we generated Pkd1;Wnt5a double knockout mice. Loss of Wnt5a suppressed phenotypes seen in the lymphatic vasculature of Pkd1(-/-) mice and Pkd1 deletion suppressed phenotypes observed in Wnt5a(-/-) mice. Thus, we report mutually suppressive roles for Pkd1 and Wnt5a, with developing lymphatic networks restored to a more wild type state in double mutant mice. This genetic interaction between Pkd1 and the non-canonical WNT signaling pathway ultimately controls LEC polarity and the morphogenesis of developing vessel networks. CONCLUSION: Our work suggests that Pkd1 acts at least in part by regulating non-canonical WNT signaling during the formation of lymphatic vascular networks.
Keywords: Animals; *Lymphatic Vessels/metabolism; Mice; Mice, Knockout; Morphogenesis/genetics; *Polycystic Kidney Diseases/genetics/metabolism; Protein Kinase C; Receptor Protein-Tyrosine Kinases/metabolism; Wnt Signaling Pathway/genetics; Wnt-5a Protein/genetics/metabolism; Pc1; Wnt5a; lymphangiogenesis; planar cell polarity; polycystic kidney disease; polycystin 1; vascular
Department(s): Laboratory Research
PubMed ID: 34174014
Publisher's Version: https://doi.org/10.1002/dvdy.390
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2024-10-16 03:58:14

Last Modified: 2024-10-16 03:59:23