SFPQ-ABL1 and BCR-ABL1 use different signaling networks to drive B-cell acute lymphoblastic leukemia
- Author(s)
- Brown, LM; Hediyeh-Zadeh, S; Sadras, T; Huckstep, H; Sandow, JJ; Bartolo, RC; Kosasih, HJ; Davidson, NM; Schmidt, B; Bjelosevic, S; Johnstone, R; Webb, AI; Khaw, SL; Oshlack, A; Davis, MJ; Ekert, PG;
- Details
- Publication Year 2022-04-12,Volume 6,Issue #7,Page 2373-2387
- Journal Title
- Blood Advances
- Publication Type
- Research article
- Abstract
- Philadelphia-like (Ph-like) acute lymphoblastic leukemia (ALL) is a high-risk subtype of B-cell ALL characterized by a gene expression profile resembling Philadelphia chromosome-positive ALL (Ph+ ALL) in the absence of BCR-ABL1. Tyrosine kinase-activating fusions, some involving ABL1, are recurrent drivers of Ph-like ALL and are targetable with tyrosine kinase inhibitors (TKIs). We identified a rare instance of SFPQ-ABL1 in a child with Ph-like ALL. SFPQ-ABL1 expressed in cytokine-dependent cell lines was sufficient to transform cells and these cells were sensitive to ABL1-targeting TKIs. In contrast to BCR-ABL1, SFPQ-ABL1 localized to the nuclear compartment and was a weaker driver of cellular proliferation. Phosphoproteomics analysis showed upregulation of cell cycle, DNA replication, and spliceosome pathways, and downregulation of signal transduction pathways, including ErbB, NF-kappaB, vascular endothelial growth factor (VEGF), and MAPK signaling in SFPQ-ABL1-expressing cells compared with BCR-ABL1-expressing cells. SFPQ-ABL1 expression did not activate phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling and was associated with phosphorylation of G2/M cell cycle proteins. SFPQ-ABL1 was sensitive to navitoclax and S-63845 and promotes cell survival by maintaining expression of Mcl-1 and Bcl-xL. SFPQ-ABL1 has functionally distinct mechanisms by which it drives ALL, including subcellular localization, proliferative capacity, and activation of cellular pathways. These findings highlight the role that fusion partners have in mediating the function of ABL1 fusions.
- Keywords
- Child; Fusion Proteins, bcr-abl/genetics/metabolism; Humans; *Phosphatidylinositol 3-Kinases/metabolism; *Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics/metabolism; Protein Kinase Inhibitors/pharmacology; Signal Transduction; Vascular Endothelial Growth Factor A
- Department(s)
- Laboratory Research
- PubMed ID
- 35061886
- Publisher's Version
- https://doi.org/10.1182/bloodadvances.2021006076
- Open Access at Publisher's Site
https://doi.org/10.1182/bloodadvances.2021006076- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-10-11 04:24:44
Last Modified: 2024-10-11 04:26:13