Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
- Author(s)
- Allam, AH; Russell, SM;
- Details
- Publication Year 2022-09-16,Volume 3,Issue #3,Page 101472
- Journal Title
- STAR Protocols
- Publication Type
- Protocol
- Abstract
- Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as beta-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).
- Keywords
- Animals; Cell Differentiation; *Lymphocyte Activation; Mice; *Receptors, Antigen, T-Cell; Signal Transduction; T-Lymphocytes; Cell Biology; Immunology; Microscopy
- Department(s)
- Laboratory Research
- PubMed ID
- 35769925
- Publisher's Version
- https://doi.org/10.1016/j.xpro.2022.101472
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-09-26 05:45:05
Last Modified: 2024-09-26 05:48:04