Repurposing Melanoma Chemotherapy to Activate Inflammasomes in the Treatment of BRAF/MAPK Inhibitor Resistant Melanoma
Details
Publication Year 2022-05,Volume 142,Issue #5,Page 1444-1455.e10
Journal Title
Journal of Investigative Dermatology
Publication Type
Research article
Abstract
The development of resistance to treatments of melanoma is commonly associated with an upregulation of the MAPK pathway and the development of an undifferentiated state. Previous studies have suggested that melanoma with these resistance characteristics may be susceptible to innate death mechanisms such as pyroptosis triggered by the activation of inflammasomes. In this study, we have taken cell lines from patients before and after the development of resistance to BRAF V600 inhibitors and exposed the resistant melanoma to temozolomide (a commonly used chemotherapy) with and without chloroquine to inhibit autophagy. It was found that melanoma with an inflammatory undifferentiated state appeared susceptible to this combination when tested in vitro and in vivo against xenografts in nonobese diabetic scid gamma mice. Translation of the latter results into patients would promise durable responses in patients treated by the combination. The inflammasome and death mechanism involved appeared to vary between melanoma and involved either AIM2 or NLRP3 inflammasomes and gasdermin D or E. These preliminary studies have raised questions as to the selectivity for different inflammasomes in different melanoma and their selective targeting by chemotherapy. They also question whether the inflammatory state of melanoma may be used as biomarkers to select patients for inflammasome-targeted therapy.
Keywords
Animals; Humans; *Inflammasomes/metabolism; *Melanoma/drug therapy/metabolism; Mice; Mice, Inbred NOD; Protein Kinase Inhibitors/pharmacology/therapeutic use; Proto-Oncogene Proteins B-raf/genetics; Pyroptosis
Department(s)
Laboratory Research
PubMed ID
34695412
Terms of Use/Rights Notice
Refer to copyright notice on published article.


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