The JNK and Hippo pathways control epithelial integrity and prevent tumor initiation by regulating an overlapping transcriptome
- Author(s)
- Mitchell, KA; Vissers, JHA; Pojer, JM; Brooks, E; Hilmi, AJS; Papenfuss, AT; Schröder, J; Harvey, KF;
- Journal Title
- Current Biology
- Publication Type
- Research article
- Abstract
- Epithelial organs maintain their integrity and prevent tumor initiation by actively removing defective cells, such as those that have lost apicobasal polarity. Here, we identify how transcription factors of two key signaling pathways-Jun-N-terminal kinase (JNK) and Hippo-regulate epithelial integrity by controlling transcription of an overlapping set of target genes. Targeted DamID experiments reveal that, in proliferating cells of the Drosophila melanogaster eye, the AP-1 transcription factor Jun and the Hippo pathway transcription regulators Yorkie and Scalloped bind to a common suite of target genes that promote organ growth. In defective neoplastic cells, AP-1 transcription factors repress transcription of growth genes together with the C-terminal binding protein (CtBP) co-repressor. If gene repression by AP-1/CtBP fails, neoplastic tumor growth ensues, driven by Yorkie/Scalloped. Thus, AP-1/CtBP eliminates defective cells and prevents tumor initiation by acting in parallel to Yorkie/Scalloped to repress expression of a shared transcriptome. These findings shed new light on the maintenance of epithelial integrity and tumor suppression.
- Publisher
- Cell Press
- Keywords
- Ap-1; CtBP; Hippo signaling; TNF/JNK signaling; Yorkie/Scalloped; epithelial integrity; neoplasia; organ growth; transcription; tumor suppression
- Department(s)
- Laboratory Research
- Publisher's Version
- https://doi.org/10.1016/j.cub.2024.07.060
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-09-19 02:48:15
Last Modified: 2024-09-19 02:49:37