Development of a hepatic cryoinjury model to study liver regeneration
- Author(s)
- Sande-Melon, M; Bergemann, D; Fernández-Lajarín, M; González-Rosa, JM; Cox, AG;
- Details
- Publication Year 2024-08-01,Volume 151,Issue #15,Page dev203124
- Journal Title
- Development
- Publication Type
- Research article
- Abstract
- The liver is a remarkable organ that can regenerate in response to injury. Depending on the extent of injury, the liver can undergo compensatory hyperplasia or fibrosis. Despite decades of research, the molecular mechanisms underlying these processes are poorly understood. Here, we developed a new model to study liver regeneration based on cryoinjury. To visualise liver regeneration at cellular resolution, we adapted the CUBIC tissue-clearing approach. Hepatic cryoinjury induced a localised necrotic and apoptotic lesion characterised by inflammation and infiltration of innate immune cells. After this initial phase, we observed fibrosis, which resolved as regeneration re-established homeostasis in 30 days. Importantly, this approach enables the comparison of healthy and injured parenchyma within an individual animal, providing unique advantages to previous models. In summary, the hepatic cryoinjury model provides a fast and reproducible method for studying the cellular and molecular pathways underpinning fibrosis and liver regeneration.
- Publisher
- The Company of Biologists
- Keywords
- *Liver Regeneration/physiology; Animals; *Liver/pathology/metabolism; Mice; Disease Models, Animal; Apoptosis; Mice, Inbred C57BL; Male; Liver Cirrhosis/pathology; Cryoinjury; Fibrosis; Inflammation; Liver regeneration; Necrosis; Proliferation; Zebrafish
- Department(s)
- Laboratory Research
- Publisher's Version
- https://doi.org/10.1242/dev.203124
- Open Access at Publisher's Site
https://doi.org/10.1242/dev.203124- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-09-03 07:46:51
Last Modified: 2024-09-03 07:49:20