Flow cytometric analysis of myelodysplasia: Pre-analytical and technical issues-Recommendations from the European LeukemiaNet

van der Velden, VHJ; Preijers, F; Johansson, U; Westers, TM; Dunlop, A; Porwit, A; Bene, MC; Valent, P; Te Marvelde, J; Wagner-Ballon, O; Oelschlaegel, U; Saft, L; Kordasti, S; Ireland, R; Cremers, E; Alhan, C; Duetz, C; Hobo, W; Chapuis, N; Fontenay, M; Bettelheim, P; Eidenshink-Brodersen, L; Font, P; Loken, MR; Matarraz, S; Ogata, K; Orfao, A; Psarra, K; Subira, D; Wells, DA; Della Porta, MG; Burbury, K; Bellos, F; Weiss, E; Kern, W; van de Loosdrecht, A

Author(s): van der Velden, VHJ; Preijers, F; Johansson, U; Westers, TM; Dunlop, A; Porwit, A; Bene, MC; Valent, P; Te Marvelde, J; Wagner-Ballon, O; Oelschlaegel, U; Saft, L; Kordasti, S; Ireland, R; Cremers, E; Alhan, C; Duetz, C; Hobo, W; Chapuis, N; Fontenay, M; Bettelheim, P; Eidenshink-Brodersen, L; Font, P; Loken, MR; Matarraz, S; Ogata, K; Orfao, A; Psarra, K; Subira, D; Wells, DA; Della Porta, MG; Burbury, K; Bellos, F; Weiss, E; Kern, W; van de Loosdrecht, A;
Details: Publication Year 2023-01,Volume 104,Issue #1,Page 15-26
Journal Title: Cytometry Part B: Clinical Cytometry
Publication Type: Research article
Abstract: BACKGROUND: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures. METHODS: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. RESULTS: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. CONCLUSIONS: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.
Publisher: Wiley
Keywords: Humans; Flow Cytometry/methods; *Myelodysplastic Syndromes/diagnosis; Reference Standards; Biological Assay; Fluorescent Dyes; Eln; Mds; flow cytometry; pre-analytic issues
Department(s): Clinical Haematology
PubMed ID: 34894176
Publisher's Version: https://doi.org/10.1002/cyto.b.22046
Terms of Use/Rights Notice: Refer to copyright notice on published article.

Creation Date: 2023-06-15 07:24:51

Last Modified: 2024-07-30 02:07:57