Neural progenitor-derived Apelin controls tip cell behavior and vascular patterning
Details
Publication Year 2024-07-05,Volume 10,Issue #27,Page eadk1174
Journal Title
Science Advances
Publication Type
Research article
Abstract
During angiogenesis, vascular tip cells guide nascent vascular sprouts to form a vascular network. Apelin, an agonist of the G protein-coupled receptor Aplnr, is enriched in vascular tip cells, and it is hypothesized that vascular-derived Apelin regulates sprouting angiogenesis. We identify an apelin-expressing neural progenitor cell population in the dorsal neural tube. Vascular tip cells exhibit directed elongation and migration toward and along the apelin-expressing neural progenitor cells. Notably, restoration of neural but not vascular apelin expression in apelin mutants remedies the angiogenic defects of mutants. By functional analyses, we show the requirement of Apelin signaling for tip cell behaviors, like filopodia formation and cell elongation. Through genetic interaction studies and analysis of transgenic activity reporters, we identify Apelin signaling as a modulator of phosphoinositide 3-kinase and extracellular signal-regulated kinase signaling in tip cells in vivo. Our results suggest a previously unidentified neurovascular cross-talk mediated by Apelin signaling that is important for tip cell function during sprouting angiogenesis.
Publisher
American Association for the Advancement of Science
Keywords
Animals; *Apelin/metabolism/genetics; *Neural Stem Cells/metabolism/cytology; *Neovascularization, Physiologic; *Signal Transduction; Zebrafish; Intercellular Signaling Peptides and Proteins/metabolism/genetics; Cell Movement; Neural Tube/metabolism; Phosphatidylinositol 3-Kinases/metabolism; Chemokines; Zebrafish Proteins
Department(s)
Laboratory Research
Open Access at Publisher's Site
https://doi.org/10.1126/sciadv.adk1174
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2024-08-06 08:21:52
Last Modified: 2024-08-06 08:24:57

© 2024 The Walter and Eliza Hall Institute of Medical Research. Access to this website is subject to our Privacy Policy and Terms of Use

An error has occurred. This application may no longer respond until reloaded. Reload 🗙