Neural progenitor-derived Apelin controls tip cell behavior and vascular patterning
- Author(s)
- Malchow, J; Eberlein, J; Li, W; Hogan, BM; Okuda, KS; Helker, CSM;
- Details
- Publication Year 2024-07-05,Volume 10,Issue #27,Page eadk1174
- Journal Title
- Science Advances
- Publication Type
- Research article
- Abstract
- During angiogenesis, vascular tip cells guide nascent vascular sprouts to form a vascular network. Apelin, an agonist of the G protein-coupled receptor Aplnr, is enriched in vascular tip cells, and it is hypothesized that vascular-derived Apelin regulates sprouting angiogenesis. We identify an apelin-expressing neural progenitor cell population in the dorsal neural tube. Vascular tip cells exhibit directed elongation and migration toward and along the apelin-expressing neural progenitor cells. Notably, restoration of neural but not vascular apelin expression in apelin mutants remedies the angiogenic defects of mutants. By functional analyses, we show the requirement of Apelin signaling for tip cell behaviors, like filopodia formation and cell elongation. Through genetic interaction studies and analysis of transgenic activity reporters, we identify Apelin signaling as a modulator of phosphoinositide 3-kinase and extracellular signal-regulated kinase signaling in tip cells in vivo. Our results suggest a previously unidentified neurovascular cross-talk mediated by Apelin signaling that is important for tip cell function during sprouting angiogenesis.
- Publisher
- American Association for the Advancement of Science
- Keywords
- Animals; *Apelin/metabolism/genetics; *Neural Stem Cells/metabolism/cytology; *Neovascularization, Physiologic; *Signal Transduction; Zebrafish; Intercellular Signaling Peptides and Proteins/metabolism/genetics; Cell Movement; Neural Tube/metabolism; Phosphatidylinositol 3-Kinases/metabolism; Chemokines; Zebrafish Proteins
- Department(s)
- Laboratory Research
- Publisher's Version
- https://doi.org/10.1126/sciadv.adk1174
- Open Access at Publisher's Site
https://doi.org/10.1126/sciadv.adk1174- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-08-06 08:21:52
Last Modified: 2024-08-06 08:24:57