In vivo clonal tracking reveals evidence of haemangioblast and haematomesoblast contribution to yolk sac haematopoiesis
Journal Title
Nature Communications
Publication Type
Research article
Abstract
During embryogenesis, haematopoietic and endothelial lineages emerge closely in time and space. It is thought that the first blood and endothelium derive from a common clonal ancestor, the haemangioblast. However, investigation of candidate haemangioblasts in vitro revealed the capacity for mesenchymal differentiation, a feature more compatible with an earlier mesodermal precursor. To date, no evidence for an in vivo haemangioblast has been discovered. Using single cell RNA-Sequencing and in vivo cellular barcoding, we have unravelled the ancestral relationships that give rise to the haematopoietic lineages of the yolk sac, the endothelium, and the mesenchyme. We show that the mesodermal derivatives of the yolk sac are produced by three distinct precursors with dual-lineage outcomes: the haemangioblast, the mesenchymoangioblast, and a previously undescribed cell type: the haematomesoblast. Between E5.5 and E7.5, this trio of precursors seeds haematopoietic, endothelial, and mesenchymal trajectories.
Publisher
Nature Portfolio
Keywords
*Yolk Sac; Hematopoiesis/genetics; *Hemangioblasts; Clone Cells; Endothelium; Cell Differentiation
Department(s)
Laboratory Research
PubMed ID
36596806
Open Access at Publisher's Site
https://doi.org/10.1038/s41467-022-35744-x
Terms of Use/Rights Notice
Refer to copyright notice on published article.


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