Spatial transcriptomics and the anatomical pathologist: Molecular meets morphology
Details
Publication Year 2024-03,Volume 84,Issue #4,Page 577-586
Journal Title
Histopathology
Publication Type
Review
Abstract
In recent years anatomical pathology has been revolutionised by the incorporation of molecular findings into routine diagnostic practice, and in some diseases the presence of specific molecular alterations are now essential for diagnosis. Spatial transcriptomics describes a group of technologies that provide up to transcriptome-wide expression profiling while preserving the spatial origin of the data, with many of these technologies able to provide these data using a single tissue section. Spatial transcriptomics allows expression profiling of highly specific areas within a tissue section potentially to subcellular resolution, and allows correlation of expression data with morphology, tissue type and location relative to other structures. While largely still research laboratory-based, several spatial transcriptomics methods have now achieved compatibility with formalin-fixed paraffin-embedded tissue (FFPE), allowing their use in diagnostic tissue samples, and with further development potentially leading to their incorporation in routine anatomical pathology practice. This mini review provides an overview of spatial transcriptomics methods, with an emphasis on platforms compatible with FFPE tissue, approaches to assess the data and potential applications in anatomical pathology practice.
Publisher
Wiley
Keywords
Humans; *Pathologists; Paraffin Embedding/methods; *Gene Expression Profiling/methods; Transcriptome; Formaldehyde/metabolism; anatomical pathology; spatial transcriptomics; surgical pathology
Department(s)
Pathology; Medical Oncology; Laboratory Research
Open Access at Publisher's Site
https://doi.org/10.1111/his.15093
Terms of Use/Rights Notice
Refer to copyright notice on published article.


Creation Date: 2024-04-16 01:22:39
Last Modified: 2024-04-16 01:23:00

© 2024 The Walter and Eliza Hall Institute of Medical Research. Access to this website is subject to our Privacy Policy and Terms of Use

An error has occurred. This application may no longer respond until reloaded. Reload 🗙