FixNCut: single-cell genomics through reversible tissue fixation and dissociation
- Author(s)
- Jiménez-Gracia, L; Marchese, D; Nieto, JC; Caratù, G; Melón-Ardanaz, E; Gudiño, V; Roth, S; Wise, K; Ryan, NK; Jensen, KB; Hernando-Momblona, X; Bernardes, JP; Tran, F; Sievers, LK; Schreiber, S; van den Berge, M; Kole, T; van der Velde, PL; Nawijn, MC; Rosenstiel, P; Batlle, E; Butler, LM; Parish, IA; Plummer, J; Gut, I; Salas, A; Heyn, H; Martelotto, LG;
- Details
- Publication Year 2024-03-29,Volume 25,Issue #1,Page 81
- Journal Title
- Genome Biology
- Publication Type
- Research article
- Abstract
- The use of single-cell technologies for clinical applications requires disconnecting sampling from downstream processing steps. Early sample preservation can further increase robustness and reproducibility by avoiding artifacts introduced during specimen handling. We present FixNCut, a methodology for the reversible fixation of tissue followed by dissociation that overcomes current limitations. We applied FixNCut to human and mouse tissues to demonstrate the preservation of RNA integrity, sequencing library complexity, and cellular composition, while diminishing stress-related artifacts. Besides single-cell RNA sequencing, FixNCut is compatible with multiple single-cell and spatial technologies, making it a versatile tool for robust and flexible study designs.
- Publisher
- BioMed Central
- Keywords
- Humans; Animals; Mice; Tissue Fixation/methods; Reproducibility of Results; Sequence Analysis, RNA/methods; *RNA/genetics; *Genomics/methods; Single-Cell Analysis/methods; Cellular stress; RNA sequencing; Sample fixation; Single-cell genomics; Tissue dissociation
- Department(s)
- Laboratory Research
- Publisher's Version
- https://doi.org/10.1186/s13059-024-03219-5
- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2024-04-04 02:32:03
Last Modified: 2024-04-04 03:11:41