Basal spot junctions of Drosophila epithelial tissues respond to morphogenetic forces and regulate Hippo signaling
Details
Publication Year 2024-01-22,Volume 59,Issue #2,Page 262-279.e6
Journal Title
Developmental Cell
Publication Type
Research article
Abstract
Organ size is controlled by numerous factors including mechanical forces, which are mediated in part by the Hippo pathway. In growing Drosophila epithelial tissues, cytoskeletal tension influences Hippo signaling by modulating the localization of key pathway proteins to different apical domains. Here, we discovered a Hippo signaling hub at basal spot junctions, which form at the basal-most point of the lateral membranes and resemble adherens junctions in protein composition. Basal spot junctions recruit the central kinase Warts via Ajuba and E-cadherin, which prevent Warts activation by segregating it from upstream Hippo pathway proteins. Basal spot junctions are prominent when tissues undergo morphogenesis and are highly sensitive to fluctuations in cytoskeletal tension. They are distinct from focal adhesions, but the latter profoundly influences basal spot junction abundance by modulating the basal-medial actomyosin network and tension experienced by spot junctions. Thus, basal spot junctions couple morphogenetic forces to Hippo pathway activity and organ growth.
Publisher
Cell Press
Keywords
E-cadherin; Hippo signaling; Warts kinase; basal spot junctions; cell adhesion; focal adhesions; growth control; mechanobiology; morphogenesis; myosin
Department(s)
Laboratory Research
Terms of Use/Rights Notice
Refer to copyright notice on published article.


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