The in situ transcriptomic landscape of breast tumour-associated and normal adjacent endothelial cells
Details
Publication Year 2024-12-06,Volume 1870,Issue #2,Page 166985
Journal Title
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Publication Type
Research article
Abstract
BACKGROUND AND AIMS: Triple Negative Breast Cancer (TNBC) is associated with increased angiogenesis, which is known to aid tumour growth and metastasis. Anti-angiogenic therapies that have been developed to target this feature have mostly generated disappointing clinical results. Further research into targeted approaches is limited by a lack of understanding of the in situ molecular profile of tumour-associated vasculature. In this study, we aimed to understand the differences in the molecular profiles of tumour endothelial cells vs normal-adjacent endothelial cells in TNBC tissues. METHOD: We have applied unbiased whole transcriptome spatial profiling of in situ gene expressions of endothelial cells localized in full-face patient TNBC tissues (n = 4) and normal-adjacent regions of the same patient breast tissues. RESULTS: Our comparative analysis revealed that 2412 genes were differentially expressed (p(adj) < 0.05) between the tumour endothelial cells and normal-adjacent endothelial cells. Pathway enrichment showed the enrichment of gene sets related to cell-cell, cell-ECM adhesion, chromatin organization and remodeling, and protein-DNA complex subunit organization. CONCLUSION: Overall, the results revealed unique molecular profiles and signalling pathways of tumour-associated vasculature, which is a critical step towards larger cohort studies investigating potential targets for TNBC prognosis and anti-angiogenic treatments.
Keywords
Abnormal vasculature; Spatial profiling; Triple Negative Breast Cancer; Tumour endothelial cells; Whole transcriptome analyses
Department(s)
Pathology
Open Access at Publisher's Site
https://doi.org/10.1016/j.bbadis.2023.166985
Terms of Use/Rights Notice
Refer to copyright notice on published article.


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