Matrix Selection for the Visualization of Small Molecules and Lipids in Brain Tumors Using Untargeted MALDI-TOF Mass Spectrometry Imaging
- Author(s)
- Lu, T; Freytag, L; Narayana, VK; Moore, Z; Oliver, SJ; Valkovic, A; Nijagal, B; Peterson, AL; De Souza, DP; McConville, MJ; Whittle, JR; Best, SA; Freytag, S;
- Details
- Publication Year 2023-11-09,Volume 13,Issue #11,Page 1139
- Journal Title
- Metabolites
- Publication Type
- Research article
- Abstract
- Matrix-assisted laser desorption/ionization mass spectrometry imaging allows for the study of metabolic activity in the tumor microenvironment of brain cancers. The detectable metabolites within these tumors are contingent upon the choice of matrix, deposition technique, and polarity setting. In this study, we compared the performance of three different matrices, two deposition techniques, and the use of positive and negative polarity in two different brain cancer types and across two species. Optimal combinations were confirmed by a comparative analysis of lipid and small-molecule abundance by using liquid chromatography-mass spectrometry and RNA sequencing to assess differential metabolites and enzymes between normal and tumor regions. Our findings indicate that in the tumor-bearing brain, the recrystallized α-cyano-4-hydroxycinnamic acid matrix with positive polarity offered superior performance for both detected metabolites and consistency with other techniques. Beyond these implications for brain cancer, our work establishes a workflow to identify optimal matrices for spatial metabolomics studies.
- Keywords
- Maldi-tof; atmospheric pressure; brain cancer; glioma; matrix selection
- Department(s)
- Medical Oncology
- Publisher's Version
- https://doi.org/10.3390/metabo13111139
- Open Access at Publisher's Site
https://doi.org/10.3390/metabo13111139- Terms of Use/Rights Notice
- Refer to copyright notice on published article.
Creation Date: 2023-12-20 05:40:25
Last Modified: 2023-12-20 06:31:07